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Bacterial Multidrug ExportersMethods and Protocols
Author(s): Akihito Yamaguchi, Kunihiko Nishino
Format: Paperback
Publisher: Humana Press Inc., United States
Imprint: Humana Press Inc.
ISBN-13: 9781493984909, 978-1493984909
Synopsis
This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria. Beginning with protocols for the structural analysis of bacterial multidrug exporters, the book continues with sections on biochemical and bioengineering analysis, computational analysis, biomedical approaches, as well as advanced technologies expected to be applied to multidrug efflux transport studies. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,Bacterial Multidrug Exporters: Methods and Protocols serves as an ideal guide to this fastmoving field of study.